mtt cell proliferation assay kit Search Results


cytotoxicity assay kit  (Beyotime)
96
Beyotime cytotoxicity assay kit
Cytotoxicity Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt cell proliferation assay kit  (Novus Biologicals)
90
Novus Biologicals mtt cell proliferation assay kit
Mtt Cell Proliferation Assay Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt cell proliferation assay kit  (R&D Systems)
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R&D Systems mtt cell proliferation assay kit
Mtt Cell Proliferation Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay  (Alphabioregen INC)
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Alphabioregen INC mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay
Mtt (3 (4,5 Dimethylthiazol 2 Yl) 2,5 Diphenyltetrazolium Bromide) Tetrazolium Reduction Assay, supplied by Alphabioregen INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell metertm colorimetric mtt cell proliferation kit  (Biomol GmbH)
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Biomol GmbH cell metertm colorimetric mtt cell proliferation kit
Cell Metertm Colorimetric Mtt Cell Proliferation Kit, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt cell proliferation kit  (OZ Biosciences)
90
OZ Biosciences mtt cell proliferation kit
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Mtt Cell Proliferation Kit, supplied by OZ Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a 96-nonradioactive-cell proliferation assay (mtt) kit  (Promega)
90
Promega a 96-nonradioactive-cell proliferation assay (mtt) kit
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
A 96 Nonradioactive Cell Proliferation Assay (Mtt) Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt cell viability and proliferation assay kit  (ScienCell)
90
ScienCell mtt cell viability and proliferation assay kit
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Mtt Cell Viability And Proliferation Assay Kit, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt-based test ez4 u cell proliferation and cytotoxicity assay kit  (Biomedica Medizinprodukte GmbH)
90
Biomedica Medizinprodukte GmbH mtt-based test ez4 u cell proliferation and cytotoxicity assay kit
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Mtt Based Test Ez4 U Cell Proliferation And Cytotoxicity Assay Kit, supplied by Biomedica Medizinprodukte GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytotoxicity mtt kit celltiter 96 non-radioactive cell proliferation assay  (Promega)
90
Promega cytotoxicity mtt kit celltiter 96 non-radioactive cell proliferation assay
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Cytotoxicity Mtt Kit Celltiter 96 Non Radioactive Cell Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(mtt viability assay kit, celltiter 96 ® aqueous non-radioactive cell proliferation assay  (Promega)
90
Promega (mtt viability assay kit, celltiter 96 ® aqueous non-radioactive cell proliferation assay
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
(Mtt Viability Assay Kit, Celltiter 96 ® Aqueous Non Radioactive Cell Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell proliferation assay kit (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide, [mtt]  (HiMedia Laboratories)
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HiMedia Laboratories cell proliferation assay kit (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide, [mtt]
The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral <t>proliferation</t> was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the <t>MTT</t> Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Cell Proliferation Assay Kit (3 (4,5 Dimethylthiazol 2 Yl) 2,5diphenyltetrazoliumbromide, [Mtt], supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral proliferation was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the MTT Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Frontiers in Immunology

Article Title: The Hypothiocyanite and Amantadine Combination Treatment Prevents Lethal Influenza A Virus Infection in Mice

doi: 10.3389/fimmu.2022.859033

Figure Lengend Snippet: The AMT+OSCN − combination treatment significantly reduces the replication of several influenza A virus strains in vitro . (A) Confluent monolayers of MDCK cells were inoculated with 100 MOI of the indicated six H1N1 and H3N2 IAV strains individually. The overlay media were supplemented with the indicated antiviral compounds. Viral proliferation was measured by PFU assay. OSL phosphate was used as a control. Data are mean ± SEM of n = 6 (A/California/04/2009, A/PR/8/34, and A/Aichi/2/1968), n = 4 (A/Texas/50/12 and A/Hong Kong/8/68), and n = 5 (A/Wisconsin/67/2005) independent experiments. One-way ANOVA and Tukey’s multiple comparisons tests. (B) Viral mRNA levels were measured under the exact same conditions and at the same time as in panel (A) by qRT-PCR for the indicated viral strains. All shown results were normalized and compared to housekeeping genes as control. OSL was used as positive control. Mean ± SEM, n = 3. One-way ANOVA and Tukey’s multiple comparisons test. (C) MDCK cells were exposed to the indicated treatment options without viral infection, and cell toxicity was measured by the MTT Cell Proliferation Kit (colorimetric assay). At 3 days after drug addition, the treatment was added at maximum dose: OSL at 5 μg/ml and AMT at 15 μg/ml. Untreated but infected cells were used as positive control for cell toxicity, while uninfected, untreated cells were used as negative control (n = 3). AMT, amantadine; MOI, multiplicity of infection; OSCN − , hypothiocyanite; OSL, oseltamivir; PFU, plaque-forming unit; MDCK, Madin–Darby canine kidney; IAV, influenza A virus.*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: The potential cytotoxicity of the tested antiviral compounds was evaluated using the MTT Cell Proliferation Kit (OZ Biosciences, Catalog # MT01000).

Techniques: Virus, In Vitro, Control, Quantitative RT-PCR, Positive Control, Infection, MTT Cell Proliferation, Colorimetric Assay, Negative Control